Feb 15, 2010 · In total, 8 ml of strain I5 was incubated at 35 °C in 500 ml reagent bottles containing 100 ml sterilized MP medium with 400 mg L −1 phenol, and 100 ml of one of the four collected supernatants (I8-E, I8-S, I5 + I8-12 h, and I5 + I8-24 h). Each set of bottles fed with a specific supernatant was further divided into three groups.