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May 16, 2016 · I dilute each vial so that each shot is .20 ml of liquid. this means .20ml per shot X 20 shots will be a total of 4ml of bacteriostatic water. You can play with these numbers to suit your taste. If you want 500mcg that means each vial will yield 10 applications. Use only 2ml of water with shots of .20ml each.