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8. ChangeTo LC/MS injection tool 9. Inject To LC-MS/MS 10. Change To prep syringe for next sample 11. Proceed With prep-ahead for next extract sample upon Ready Signal. Table 2. Steps for automated online μSPE clean-up method with LC injection. For the purpose of comparison, dispersive SPE was performed using two different blends of sorbents.

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LC-MS/MS analysis The LC-MS/MS system comprised a Vanquish Flex UHPLC binary system interfaced with a TSQ Altis triple quadrupole mass spectrometer equipped with a H-ESI ionization probe. Chromatographic separation was carried out on a Thermo Scientific ™ Accucore VDX column, 100 mm × 2.1 mm × 2.6 μm (P/N VDX-102130) at a temperature of

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The LC-MS/MS system used for determination and quantification of the analytes was a Vanquish ultra-high pressure liquid chromatograph (UHPLC, Thermo Fisher, San Jose, CA) coupled to a high-performance, ion funnel TSQ Altis ™ Triple Quadrupole Mass Spectrometer (Thermo Fisher, San Jose, CA). Analytes were separated with an Acclaim® Mixed-Mode