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17. Discard the collection tube and place the column into a clean 1.5 ml tube, add 50 μl RNase-free water to the center of the membrane, incubate 1 min at room temperature and centrifuge for 1 min at 10,000 rpm. 18. RNA can be used immediately or stored at 70 C. 3.3.2 Quantification and RNA Integrity Check 1.