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In order to get better recovery in the solution, do following: (1). try to minimize both the surface interaction by using low binding material and the surface area exposed to the solution; and (2

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Nov 02, 2018 · Dynamic binding capacity (DBC) is the binding capacity under operating conditions (i.e., in a packed affinity chromatography column during sample application). The DBC of a chromatography resin is the amount of target protein that binds to the resin under given flow conditions before a significant breakthrough of unbound protein

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order autosampler glass vials low protein binding-HPLC Dec 22, 2021 · 11 mm Snap Top Clear Glass Vial with PTFE/Silicone Septa, 100/pk 22 12 2021 The PerkinElmer 2 mL, 11 mm Crimp Top Vial with PTFE/Silicone Closure- Kit These autosampler vial, cap and septa kits make it surprisingly simple to

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Feb 14, 2014 · Dried protein digests were dissolved in 20 μl of 1% formic acid, centrifuged (10 000 × g, 5 min, 4°C) and the supernatant transferred to inserts in vials. The nano-HPLC apparatus used for protein digests analysis was a Proxeon Easy-nLC (Proxeon, Odense, Denmark) coupled to a maXis Q-TOF (quadrupole – time of flight) mass spectrometer with

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Jan 01, 2022 · Transfer 75 μL from the tubes to HPLC vials. Add 125 μL DDW to each vial to adjust the volume to 200 μL and close vials with a snap cap. • Analyze the nucleoside content using an HPLC: o. In separate runs, inject 125 μL from each vial on a C18 reverse phase column equilibrated in 98% HPLC buffer A and 2% HPLC buffer B. o

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When to use snap caps vs. screw caps - MicroSolv Technology Oct 29, 2013 Screw caps on the other hand provide a better seal (when the cap is properly Autosampler Vials, Caps

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