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Determination of CBD, 7-hydroxy, and 7-carboxy metabolites in
An HTC PAL autosampler (Leap Technologies, NC) was used to inject 20 µL of each sample onto an / Series Aijiren HPLC system. Samples were maintained at 10° C in the autosampler. The mass spectrometer was an API 4000 with Turboionspray source and using Analyst software, v. 1.6.2 (AB Sciex).
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The CTC PAL sample injectors enable GC and GC/MS laboratories to increase productivity with greater automated sample preparation capabilities and a variety of sample vial options. The high-end CombiPAL sample injector offers three types of sample injection techniques: liquid injection, headspace, and solid-phase microextraction (SPME).
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Step 1. Preparation of Solvents Arium ® Mini Plus UV and Arium ® Pro ultrapure lab water systems Learn More Step 2. Preparation of Standards Cubis ® balance with Q-App software Learn More Step 3. Pipetting Tacta ® and Picus ® pipettes with Low Retention tips Learn More Step 4. Filtration Claristep ® filtration system and Minisart ® filtration units
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Filtration is an essential sample preparation step before sensitive chromatographic analysis, such as HPLC and LC-MS. Particulates in samples can interfere with Sample Preparation for HPLC If samples are refrozen, they must be filtered through new microcentrifuge filter tubes prior to HPLC analysis. 5. Protein analysis a. Remove all residual
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2. SAMPLE PREPARTAION IN HPLC Introduction: Sample preparation is an essential part of HPLC analysis intended to provide a reproducible and homogenous solution that is suitable for injection on to the column. Aim of sample preparation: Should be free of interferences; will not damage the column; compatible with HPLC method.
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