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A constant application rate of 0.1µLs-1 was employed, and space between the two bands was kept constant at 10 mm. The plates were developed in a linear ascending mode using toluene-chloroform-methanol-acetic acid (2.0:2.0:1.0:0.04, v/v/v) as mobile phase in a twin through glass chambers previously saturated with mobile phase for 30 min at room

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