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The mixture was then shaken vigorously for 1 min and stranded for stratification. An aliquot from the upper layer was evaporated to near dryness with a vacufuge concentrator (Eppendorf, Hamburg, Germany) at 30 °C. The residual extract was dissolved in acetonitrile, filtered through a 0.2-μm filter, and placed in a 2-mL vial for HPLC analysis.

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