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Protein binding can enhance or detract from a drug's performance. As a general rule, agents that are minimally protein bound penetrate tissue better than those that are highly bound, but they are excreted much faster. Among drugs that are less than 80-85 percent protein bound, differences appear to be of slight clinical importance.

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The magnitude of a protein's C 1/2 SPROX value shift will depend on the binding free energy, which is a function of both the inherent affinity of the protein-ligand binding interaction(s) responsible for the hit and the free ligand concentration used in the SPROX analysis. 5 In our proof-of-principle studies, a C 1/2 SPROX value shift of 1.0 M

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